Single cell RNA sequencing of microglia throughout the mouse
lifespan and in the injured brain reveals complex cell-state changes
Microglia are the resident immune cells of the brain and are highly
dynamic cells that respond to changes in their environment. We
sequenced mouse microglia from E14.5 to 18 months as well as following
demyelinating injury to capture the most comprehensive atlas of
microglia cell state diversity to date. Use this searchable database
to explore these state changes and gene expression changes in over
76,000 sequenced microglia from 41 mice. Download cluster-specific
markers
here.
Methods
It was essential to isolate microglia without activating them ex vivo.
We developed a novel protocol to isolate microglia at 4 degrees to
minimize any potential artifacts (see diagram). Detailed description
of methods in published manuscript.
Manuscript
Hammond TR, Dufort C, Dissing-Olesen L, Giera S, Young A, Wysoker A,
Walker A, Segel M, Saunders A, Macosko E, Marsh SM, Nemesh J, Ginhoux
F, Chen J, Franklin RJ, Piao X, McCarroll S, and Stevens B.
Single cell RNA sequencing of microglia throughout the mouse
lifespan and in the injured brain reveals complex cell-state
changes. Immunity (2018).
